Coding

Part:BBa_K1916013:Design

Designed by: Jonathan Chen   Group: iGEM16_UC_Davis   (2016-10-11)


CBCR3 + intein-CBD tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 513
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 1126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.


Source

Metagenomic data mining (JGI)

References